As filed with the Securities and Exchange Commission on June 11, 2001
                                                      Registration No. 333-59324
================================================================================

                      SECURITIES AND EXCHANGE COMMISSION
                            Washington, D.C. 20549
                                _______________

                                Amendment No. 1
                                      to
                                   FORM S-3
                            REGISTRATION STATEMENT
                                     UNDER
                          THE SECURITIES ACT OF 1933

                                AMERIPATH, INC.
            (Exact name of registrant as specified in its charter)

          Delaware                                          65-0642485
(State or other jurisdiction of                          (I.R.S. Employer
incorporation or organization)                        Identification Number)


                                 James C. New
                            Chief Executive Officer
                                AmeriPath, Inc.
                          7289 Garden Road, Suite 200
                         Riviera Beach, Florida 33404
                           Telephone: (561) 845-1850
                           Facsimile: (561) 845-0129
         (Address, including zip code, and telephone number, including
 area code, of registrant's principal executive offices and agent for service)

     The Commission is requested to send copies of all communications to:

                            J. Vaughan Curtis, Esq.
                               Alston & Bird LLP
                          1201 West Peachtree Street
                          Atlanta, Georgia 30309-3424
                           Telephone: (404) 881-7000
                           Facsimile: (404) 881-7777



     Approximate date of commencement of proposed sale to the public: As soon as
practicable after the Registration Statement becomes effective.
     If the only securities being registered on this Form are being offered
pursuant to dividend or interest reinvestment plans, please check the following
box. [_]
     If any of the securities being registered on this Form are to be offered on
a delayed or continuous basis pursuant to Rule 415 under the Securities Act of
1933, other than securities offered only in connection with dividend or interest
reinvestment plans, check the following box. [X]
     If this Form is filed to register additional securities for an offering
pursuant to Rule 462(b) under the Securities Act, please check the following box
and list the Securities Act registration statement number of the earlier
effective registration statement for the same offering. [_]
     If this Form is a post-effective amendment filed pursuant to Rule 462(c)
under the Securities Act, check the following box and list the Securities Act
registration statement number of the earlier effective registration statement
for the same offering. [_]
     If delivery of the prospectus is expected to be made pursuant to Rule 434,
please check the following box. [_]

                              ___________________

     The registrant hereby amends this registration statement on such date or
dates as may be necessary to delay its effective date until the registrant shall
file a further amendment which specifically states that this registration
statement shall thereafter become effective in accordance with Section 8(a) of
the Securities Act of 1933 or until this registration statement shall become
effective on such date as the Commission, acting pursuant to said Section 8(a),
may determine.

================================================================================



The information in this prospectus is not complete and may be changed. The
selling stockholders may not sell these securities until the registration
statement relating to these securities that we have filed with the Securities
and Exchange Commission is effective. This prospectus is not an offer to sell
these securities and it is not soliciting an offer to buy these securities in
any state where the offer or sale is not permitted.

                   Subject to Completion, Dated June 11, 2001

PROSPECTUS

                                864,849 Shares

                                AMERIPATH, INC.

                                 Common Stock

     The stockholders named in the table included in the "Selling Stockholders"
section of this prospectus, which begins on page 13, are offering and selling up
to 864,849 shares of our common stock under this prospectus.

     Of the 864,849 shares covered by this prospectus, 859,764 shares are
presently issued and outstanding and 5,085 shares have been reserved for
issuance pursuant to the exercise of warrants held by some of the selling
stockholders. Those selling stockholders must first exercise the warrants and
acquire the underlying shares from us before they can resell those shares under
this prospectus.

          Our common stock is listed on the Nasdaq National Market under the
symbol "PATH". On June 7, 2001, the last sale price of our common stock as
reported by Nasdaq was $26.25 per share.

                           _____________________

     This investment involves risks. See "Risk Factors" beginning on page 3.

                           _____________________

     Neither the Securities and Exchange Commission nor any state commission has
approved or disapproved of these securities or determined if this prospectus is
truthful or complete. Any representation to the contrary is a criminal offense.


             The date of this prospectus is ___________ ___, 2001


                               TABLE OF CONTENTS



                                                                          PAGE
                                                                        
PROSPECTUS SUMMARY........................................................   3
RISK FACTORS..............................................................   3
CAUTIONARY STATEMENT CONCERNING FORWARD-LOOKING STATEMENTS................  11
USE OF PROCEEDS...........................................................  12
SELLING STOCKHOLDERS......................................................  13
PLAN OF DISTRIBUTION......................................................  17
LEGAL MATTERS.............................................................  18
EXPERTS...................................................................  18
WHERE YOU CAN FIND MORE INFORMATION.......................................  18
INCORPORATION OF CERTAIN DOCUMENTS BY REFERENCE...........................  19



                                       2


                              PROSPECTUS SUMMARY

     We are the largest physician and laboratory company focused on providing
anatomic pathology, cancer diagnostics, genomics, and healthcare information
services. Since the first quarter of 1996, we have completed the acquisition of
49 physician practices located in 21 states. These practices are either directly
owned or managed by us through one of our subsidiaries. Our 425 pathologists
provide medical diagnostic services in outpatient laboratories owned, operated
and managed by us, and in hospitals and outpatient ambulatory surgery centers.
Of these pathologists, 419 are board certified in anatomic and clinical
pathology, and 190 are also board certified in a subspecialty of anatomic
pathology, including dermatopathology (study of diseases of the skin),
hematopathology (study of diseases of the blood) and cytopathology (study of
abnormalities of the cells).

     On November 30, 2000, we consummated a merger with Inform DX in which we
(1) issued an aggregate of approximately 2.6 million shares of our common stock
in exchange for all of the outstanding shares of capital stock of Inform DX and
(2) assumed certain outstanding stock options and warrants of Inform DX. We also
granted the former stockholders and warrant holders of Inform DX rights to
register for resale up to one-third of the shares of our common stock they
received in the merger or have the right to receive pursuant to the warrants we
assumed. We have prepared this prospectus and registered the shares offered by
the selling stockholders in order to comply with these registration rights. The
selling stockholders acquired all of the shares of our common stock that they
are offering under this prospectus or warrants to purchase such shares in
connection with the Inform DX merger.

     Our principal executive offices are located at 7289 Garden Road, Suite 200,
Riviera Beach, Florida 33404. Our telephone number is (561) 845-1850. Our
Internet address is www.ameripath.com. The information contained on our web site
is not part of this prospectus.

                                 RISK FACTORS

     Any investment in our common stock involves a high degree of risk. You
should carefully consider each of the following risks and all of the other
information set forth in this prospectus before purchasing our common stock. If
any of the following risks actually occur, our business prospects, financial
condition and results of operations could be materially adversely affected and
the trading price of our common stock could decline. In any such case, you could
lose all or part of your investment in our company.

Our business could be harmed by future interpretation or implementation of state
laws regarding prohibitions on the corporate practice of medicine.

     We acquire or affiliate with physician practices located in many states
across the country. However, the laws of many states prohibit business
corporations, including AmeriPath and its subsidiaries, from owning corporations
that employ physicians, or from exercising control over the medical judgments or
decisions of physicians. These laws and their interpretations vary from state to
state and are enforced by both the courts and regulatory authorities, each with
broad discretion. The manner in which we operate each practice is determined
primarily by the corporate practice of medicine restrictions of the state in
which the practice is located and other applicable regulations.

     We believe that we are currently in material compliance with the corporate
practice of medicine laws in each of the states in which we operate.
Nevertheless, it is possible that regulatory authorities or other parties may
assert that we are engaged in the unauthorized corporate practice of medicine.
If such a claim were successfully asserted in any jurisdiction, we could be
subject to civil and criminal penalties and could be required to restructure our
contractual and other arrangements. Any restructuring of our

                                       3


contractual and other arrangements with physician practices could result in
lower revenues from such practices, increased expenses in the operation of such
practices and reduced influence over the business decisions of such practices.
Alternatively, some of our existing contracts could be found to be illegal and
unenforceable, which could result in the termination of those contracts and an
associated loss of revenue. In addition, expansion of our operations to other
"corporate practice" states may require structural and organizational
modification to the form of relationship that we currently have with physicians,
affiliated practices and hospitals. Such modifications could result in less
profitable relationships with physicians, affiliated practices and hospitals,
less influence over the business decisions of physicians and affiliated
practices and failure to achieve our growth objectives.

We could be hurt by future interpretation or implementation of federal anti-
kickback laws.

     Federal anti-kickback laws and regulations prohibit the offer, payment,
solicitation and receipt of any form of remuneration in exchange for referrals
of products or services for which payment may be made by Medicare, Medicaid or
other federal health care programs. Violations of federal anti-kickback laws are
punishable by monetary fines, civil and criminal penalties and exclusion from
participation in Medicare, Medicaid and other federal health care programs.
Several states have similar laws.  While we believe our operations are in
material compliance with applicable Medicare and fraud and abuse laws, including
the anti-kickback law, there is a risk that the federal government might
investigate our arrangements with physicians and third parties. Such
investigations, regardless of their outcome, could damage our reputation and
adversely affect important business relationships that we have with third
parties, including physicians, hospitals and private payors.  If our
arrangements with physicians and third parties were found to be illegal, we
could be subject to civil and criminal penalties, including fines and possible
exclusion from participation in government payor programs.  Significant fines
could cause liquidity problems and adversely affect our results of operations.
Exclusion from participation in government payor programs would eliminate an
important source of revenue and adversely affect our business.

Our business could be harmed by future interpretation or implementation of the
federal Stark Law and other state and federal anti-referral laws.

     We are also subject to federal and state statutes and regulations banning
payments for referrals of patients and referrals by physicians to health care
providers with whom the physicians have a financial relationship. The federal
Stark Law applies to Medicare and Medicaid and prohibits a physician from
referring patients for certain services, including laboratory services, to an
entity with which the physician has a financial relationship.  Financial
relationship includes both investment interests in an entity and compensation
arrangements with an entity. Many states have similar laws.  These state laws
generally apply to services reimbursed by both governmental and private payors.
Violations of these federal and state laws may result in prohibition of payment
for services rendered, loss of licenses, fines, criminal penalties and exclusion
from governmental and private payor programs. We have financial relationships
with our physicians, as defined by the federal Stark Law, in the form of
compensation arrangements, ownership of our common stock and contingent
promissory notes issued by us in connection with acquisitions. While we believe
that our financial relationships with physicians are in material compliance with
applicable laws and regulations, government authorities might take a contrary
position.  If our financial relationships with physicians were found to be
illegal, we could be subject to civil and criminal penalties, including fines,
exclusion from participation in government and private payor programs and
requirements to refund amounts previously received from government and private
payors.  In addition, expansion of our operations to new jurisdictions, or new
interpretations of laws in our existing jurisdictions, could require structural
and organizational modifications of our relationships with physicians to comply
with that jurisdiction's laws. Such structural and organizational modifications
could result in lower profitability and failure to achieve our growth
objectives.

                                       4


We could be hurt by future interpretation or implementation of state and federal
anti-trust laws.

     In connection with the corporate practice of medicine laws, the physician
practices with which we are affiliated in some states are organized as separate
legal entities. As such, the physician practice entities may be deemed to be
persons separate both from us and from each other under the antitrust laws and,
accordingly, subject to a wide range of laws that prohibit anti-competitive
conduct among separate legal entities. In addition, we are seeking to acquire or
affiliate with established and reputable practices in our target geographic
markets. While we believe that we are in compliance with these laws and intend
to comply with any  laws that may apply to our development of integrated health
care delivery networks, courts or regulatory authorities could nevertheless
investigate our business practices.  If our business practices were found to
violate these laws, we could be required to pay fines, penalties and damage
awards and we could be required to restructure our business in a manner that
would reduce our profitability or impede our growth.

Our business could be harmed by future interpretation or implementation of the
Health Care Insurance Portability and Accountability Act

     The Health Care Insurance Portability and Accountability Act, or HIPAA,
created provisions that impose criminal penalties for fraud against any health
care benefit program, for theft or embezzlement involving health care and for
false statements in connection with the payment of any health benefits. The
HIPAA provisions apply not only to federal programs, but also to private health
benefit programs. HIPAA also broadened the authority of the OIG to exclude
participants from federal health care programs. Because of the uncertainties as
to how the HIPAA provisions will be enforced, we are currently unable to predict
their ultimate impact on us. Compliance with HIPAA could cause us to modify our
business operations in a manner that would increase our operating costs or
impede our growth.  In addition, although we are unaware of any current
violations of HIPAA, if we were found to be in violation of HIPPAA, the
government could seek penalties against us or seek to exclude us from
participation in government payor programs.

We charge our clients on a fee-for-service basis, so we incur financial risk
related to collections as well as potentially long collection cycles when
seeking reimbursement from third party payors.

     Substantially all of our net revenues are derived from our practices'
charging for services on a fee-for-service basis. Accordingly, we assume the
financial risk related to collection, including the potential uncollectability
of accounts, long collection cycles for accounts receivable and delays attendant
to reimbursement by third party payors, such as governmental programs, private
insurance plans and managed care organizations. Increases in write-offs of
doubtful accounts, delays in receiving payments or potential retroactive
adjustments and penalties resulting from audits by payors may adversely affect
our operating cash flow and liquidity, require us to borrow funds to meet our
current obligations, reduce our profitability, impede our growth or otherwise
adversely affect our business.

We rely upon reimbursement from government programs for a significant portion of
our revenues, and therefore our business would be harmed if reimbursement rates
from government programs decline.

     We derive approximately 20% of our collections from payments made by
government sponsored health care programs (principally Medicare and Medicaid).
These programs are subject to substantial regulation by federal and state
governments. Any changes in reimbursement regulations, policies, practices,
interpretations or statutes that place limitations on reimbursement amounts, or
changes in reimbursement coding practices, could adversely affect our business
by reducing revenues and lowering profitability. Increasing budgetary pressures
at both the federal and state level and concerns over escalating costs of health
care have led, and may continue to lead, to significant reductions in health
care

                                       5


reimbursements. State concerns over the growth in Medicaid expenditures also
could result in payment reductions. In addition, Medicare, Medicaid and other
government sponsored health care programs are increasingly shifting to forms of
managed care, which generally offer lower reimbursement rates. Some states have
enacted legislation to require that all Medicaid patients be transitioned to
managed care organizations, which could result in reduced payments to us for
such patients. Similar legislation may be enacted in other states. In addition,
a state-legislated shift of Medicaid patients to a managed care organization
could cause us to lose some or all Medicaid business in that state if we were
not selected by the managed care organization as a participating provider.
Additionally, funds received under all health care reimbursement programs are
subject to audit with respect to the proper billing for physician services and,
accordingly, retroactive adjustments of revenue from these programs could occur.
We expect that there will continue to be proposals to reduce or limit Medicare
and Medicaid reimbursements.

There has been an increasing number of state and federal investigations of
hospitals and hospital laboratories, which may increase the likelihood of
investigations of our business practices.

     Significant media and public attention has been focused on the health care
industry due to ongoing federal and state investigations reportedly related to
referral and billing practices, laboratory and home health care services and
physician ownership and joint ventures involving hospitals. Most notably, HCA is
reportedly under investigation with respect to such practices. We operate
laboratories on behalf of numerous hospitals and have numerous contractual
agreements with hospitals, including 27 pathology service contracts with HCA
hospitals as of December 31, 2000. Therefore, the government's ongoing
investigation of HCA or other hospital operators could result in governmental
investigations of one or more of our operations.  In addition, the OIG and the
Department of Justice have initiated hospital laboratory billing review projects
in certain states and are expected to extend such projects to additional states,
including states in which we operate hospital laboratories. These projects
further increase the likelihood of governmental investigations of laboratories
that we own or operate.  Although we monitor our billing practices and hospital
arrangements for compliance with prevailing industry practices under applicable
laws, such laws are complex and constantly evolving and it is possible that
governmental investigators may take positions that are inconsistent with our
practices or industry practices. The government's investigations of entities
with which we contract may have other adverse effects on us, including
termination or amendment of one or more of our contracts or the sale of
hospitals potentially disrupting the performance of services under our
contracts. In addition, some indemnity insurers and other non-governmental
payors have sought repayment from providers, including laboratories, for alleged
overpayments.

The heightened scrutiny of Medicare and Medicaid billing practices in recent
years may increase the possibility that we will become subject to costly and
time consuming investigations.

     Payors periodically reevaluate the services for which they provide
reimbursement. In some cases, government payors such as Medicare also may seek
to recoup payments previously made for services determined not to be
reimbursable. Any such action by payors would adversely affect our revenues and
earnings.  Moreover, the federal government has become more aggressive in
examining laboratory billing practices and seeking repayments and penalties
allegedly resulting from improper billing for services (e.g., the billing codes
used). While the primary focus of this initiative has been on hospital
laboratories and on routine clinical chemistry tests, which comprise only a
portion of our revenues,  the scope of this initiative could expand and it is
not possible to predict whether or in what direction the expansion might occur.
While we believe that our practices are proper and do not include any allegedly
improper practices now being examined, the government could broaden its
initiative to focus on the type of services we furnish.  If this were to happen,
we might be required to repay money.  Furthermore, HIPAA and Operation Restore
Trust have strengthened the powers of the OIG and increased the funding for
Medicare and Medicaid audits and investigations. As a result, the OIG is
currently expanding the scope of its health

                                       6


care audits and investigations. Federal and state audits and inspections,
whether on a scheduled or unannounced basis, are conducted from time to time at
our facilities. If a negative finding is made as a result of such an
investigation, we could be required to change coding practices or repay amounts
paid for incorrect practices.

We are dependent on hospital contracts for a significant portion of our
revenues, which are short term and can easily be terminated.

     Our hospital contracts typically have terms of one to five years from their
date of execution and automatically renew for additional one-year terms unless
otherwise terminated by either party. The contracts generally provide that the
hospital may terminate the agreement prior to the expiration of the initial or
any renewal term. Loss of any particular hospital contract would not only result
in a loss of net revenue to us under that contract, but may also result in a
loss of outpatient net revenue that may be derived from our relationship with
the hospital and its medical staff. Continuing consolidation in the hospital
industry may result in fewer hospitals or fewer laboratories as hospitals move
to combine their operations. As of December 31, 2000, our practices had
contracts with 224 hospitals. Our contracts with hospitals may be terminated or
may not be renewed as their current terms expire.

If we are unable to make acquisitions in the future, our rate of growth will
slow.

     Much of our historical growth has come from acquisitions, and we expect to
continue to pursue growth through the acquisition and development of
laboratories and physician practices.  However, we may be unable to continue to
identify and complete suitable acquisitions at prices we are willing to pay or
to obtain the necessary financing on acceptable terms.  In addition, as we
become a bigger company, the amount that acquired businesses contribute to our
revenue and profits will likely be smaller on a percentage basis.  We compete
with other companies to identify and complete suitable acquisitions.  We expect
this competition to intensify, making it more difficult to acquire suitable
companies on favorable terms.  Further, the businesses we acquire may not
perform well enough to justify our investment.  If we are unable to make
additional acquisitions on suitable terms, we may not meet our growth
expectations.

Our future growth will depend on our ability to secure adequate capital
resources and to effectively integrate newly acquired practices.

     In addition to acquisitions of and affiliations with practices, we intend
to continue to grow through internal expansion. We derive our net revenue from
the net revenue of our practices. Our growth strategy requires: (1) capital
investment; (2) compliance with present or future laws and regulations that may
differ from those to which we are currently subject; (3) further development of
our management, operational, financial and accounting resources to accommodate
and manage growth; and (4) the ability to expand our physician and employee base
and to train, motivate and manage employees. Failure to meet these requirements
could impede our growth objectives or adversely affect our profitability.

     Our expansion into new markets will require us to maintain and establish
payor and customer relationships and to convert the patient tracking and
financial reporting systems of new practices to our systems.  Significant delays
or expenses with regard to this process could  adversely affect the integration
of additional practices and our profitability.  The integration of additional
practices also requires the implementation and centralization of purchasing,
accounting, human resources, management information systems, cash management and
other systems, which may be difficult, costly and time-consuming.  Accordingly,
our operating results in fiscal quarters immediately following a new practice
affiliation may be adversely affected while we attempt to complete the
integration process. We may encounter significant unanticipated costs or other
problems associated with the future integration of practices into our combined
network of affiliated practices.

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We may inherit significant liabilities from practices that we acquire.

     We perform due diligence investigations with respect to potential
liabilities of acquired and affiliated practices and obtain indemnification with
respect to liabilities from the sellers of such practices.  Nevertheless,
undiscovered claims may arise and liabilities for which we become responsible
may be material and may exceed either the limitations of any applicable
indemnification provisions or the financial resources of the indemnifying
parties. While we believe, based on our due diligence investigations, that the
operations of our practices prior to their acquisition were generally in
compliance with applicable health care laws, it is nevertheless possible that
such practices were not in full compliance with such laws and that we will
become accountable for their non-compliance. A violation of such laws by a
practice could result in civil and criminal penalties, exclusion of the
physician, the practice or us from participation in Medicare and Medicaid
programs and loss of a physician's license to practice medicine.

We have significant contingent liabilities payable to many of the sellers of
practices that we have acquired.

     In connection with our practice acquisitions, we typically agree to pay the
sellers additional consideration in the form of contingent debt obligations,
payment of which depends upon the practice achieving specified profitability
criteria over periods ranging from three to five years after the acquisition.
The amount of these contingent payments cannot be determined until the
contingency periods terminate and achievement of the profitability criteria is
determined. As of December 31, 2000, if the maximum criteria for the contingency
payments with respect to all prior acquisitions were achieved, we would be
obligated to make payments, including principal and interest, of approximately
$198.4 million over the next three to five years.  Lesser amounts would be paid
if the maximum criteria are not met. Although we believe we will be able to make
such payments from internally generated funds or proceeds of future borrowings,
it is possible that such payments could cause significant liquidity problems for
us. Payments of these contingent amounts will adversely affect our earnings per
share and may cause volatility in the market price of our common stock. We
expect to continue to use contingent notes as partial consideration for
acquisitions and affiliations.

We have recorded a significant amount of intangible assets, which may never be
realized.

     Our acquisitions have resulted in significant increases in net identifiable
intangible assets and goodwill. Net identifiable intangible assets, which
include hospital contracts, physician client lists, management service
agreements and laboratory contracts acquired in acquisitions were approximately
$266.0 million at March 31, 2001, representing approximately 45.9% of our total
assets. Net identifiable intangible assets are recorded at fair value on the
date of acquisition and are being amortized over periods ranging from 10 to 40
years. Goodwill, which relates to the excess of cost over the fair value of net
assets of businesses acquired, was approximately $189.7 million at March 31,
2001, representing approximately 32.7% of our total assets. We amortize goodwill
on a straight-line basis over periods ranging from 15 to 35 years. On an ongoing
basis, we make an evaluation to determine whether events and circumstances
indicate that all or a portion of the carrying value of intangible assets may no
longer be recoverable, in which case an additional charge to earnings may be
necessary. We may not ever realize the full value of our intangible assets.  Any
future determination requiring the write-off of a significant portion of
unamortized intangible assets could adversely affect our  results of operations
for the period in which the write-off occurs, which could adversely affect our
stock price.

                                       8


Our business is highly dependent on the recruitment and retention of qualified
pathologists.

     Our business is dependent upon recruiting and retaining pathologists,
particularly those with subspecialties, such as dermatopathology. While our
practices have been able to recruit (principally through practice acquisitions)
and retain pathologists, we may be unable to continue to do so in the future as
competition for the services of pathologists increases. In addition, we may have
to modify the economic terms of our relationships with pathologists in order
enhance our recruitment and retention efforts, which could adversely affect our
profitability.  The relationship between the pathologists and their respective
local medical communities is important to the operation and continued
profitability of each practice. Loss of one of our pathologists could lead to
the loss of hospital contracts or other sources of revenue that depend on our
continuing relationship with that pathologist.  Our revenues and earnings could
be adversely affected if a significant number of pathologists terminate their
relationships with our practices or become unable or unwilling to continue their
employment, or if a number of our non-competition agreements with physicians
were terminated or determined to be invalid or unenforceable.

Proposals to reform the health care industry may restrict our existing
operations, impose additional requirements on us, limit our expansion or
increase our costs of regulatory compliance.

     Federal and state governments have recently focused significant attention
on health care reform. It is not possible to predict which, if any, proposal
will be adopted. It is possible that the health care regulatory environment will
change so as to restrict our existing operations, impose additional requirements
on us or limit our expansion. Costs of compliance with changes in government
regulations may not be subject to recovery through price increases.

Competition from other providers of pathology services may adversely affect our
business.

     Our services include the provision of physician practice management
services to pathology practices and the provision of pathology and cytology
diagnostic services.  Companies in other health care segments, such as
hospitals, national clinical laboratories, third party payors and health
maintenance organizations may compete with us in the employment of pathologists
and the management of pathology practices. We also expect to experience
increasing competition in the provision of pathology and cytology diagnostic
services  from other anatomic pathology practices, companies in other health
care industry segments (such as other hospital-based specialties), national
clinical laboratories, large physician group practices or other pathology
physician practice management companies. Some of our competitors may have
greater financial and other resources than us, which could further intensify
competition.  Increasing competition may erode our customer base and reduce our
sources of revenue and may increase our marketing and other costs of doing
business. Increasing competition may also impede our growth objectives by making
it more difficult or more expensive for us to acquire or affiliate with
additional pathology practices.

We may be subject to significant professional liability claims and we cannot
assure you that our insurance coverage limits will be sufficient to cover such
claims.

     Our business entails an inherent risk of claims of physician professional
liability for acts or omissions of our physicians and laboratory personnel. We
and our physicians periodically become involved as defendants in medical
malpractice lawsuits, some of which are currently ongoing, and are subject to
the attendant risk of substantial damage awards. While we believe that we have
an adequate risk management program, including professional liability insurance
coverage, it is possible that future claims  will exceed the limits of our risk
management program, including the limits of our insurance coverage.  It is also
possible that the costs of our insurance coverage will rise causing us to either
incur additional costs or further limit the amount of coverage we have. In
addition, our insurance does not cover

                                       9


all potential liabilities arising from governmental fines and penalties,
indemnification agreements and certain other uninsurable losses.

The continued growth of managed care may have a material adverse effect on our
business.

     The number of individuals covered under managed care contracts or other
similar arrangements has grown over the past several years and may continue to
grow in the future. Entities providing managed care coverage have been
successful in reducing payments for medical services in numerous ways, including
entering into arrangements under which payments to a service provider are
capitated, limiting testing to specified procedures, denying payment for
services performed without prior authorization and refusing to increase fees for
specified services. These trends reduce revenues, increase the cost of doing
business and limit the ability to pass cost increases on to customers.
Therefore, the continued growth of the managed care industry could adversely
affect our business.

Our business strategy emphasizes growth, which places significant demands on our
financial, operational and management resources and creates the risk of failing
to meet the growth expectations of investors.

     Our growth strategy includes efforts to acquire and develop new practices,
develop and expand managed care and national clinical lab contracts and develop
new products, services, technologies and related alliances with third parties.
The pursuit of this growth strategy consumes capital resources, thereby creating
the financial risk that we will not realize an adequate return on this
investment. In addition, our growth may involve the acquisition of companies,
the development of products or services or the creation of strategic alliances
in areas in which we do not currently operate. This would require our management
to develop expertise in new areas, manage new business relationships and attract
new types of customers. The success or failure of our growth strategies is
difficult to predict. The failure to achieve our stated growth objectives or the
growth expectations of investors could disappoint investors and harm our stock
price. We may not be able to implement our growth strategy successfully or to
manage our expanded operations effectively and profitably.

We are pursuing a strategy of becoming a fully integrated healthcare diagnostic
information provider, which adds uncertainty to our future results of operations
and could divert financial and management resources away from our core business.

     As we pursue our transition into becoming a fully integrated healthcare
diagnostic information provider, we anticipate that significant amounts of
future revenue may be derived from products, services and alliances that do not
exist today or have not been marketed in sufficient quantities to measure
accurately market acceptance.  Similarly, post-transition operating costs are
difficult to predict with accuracy, thereby adding further uncertainty to our
future results of operations.  We may experience difficulties that could delay
or prevent the successful development and introduction of new healthcare
diagnostic information products and services and such products and services may
not achieve market acceptance.  Any failure by us to complete this transition in
a timely and cost-efficient manner could result in financial losses and could
inhibit our anticipated growth.  In addition, the pursuit of this transition
could divert financial and management resources away from our core
business.

                                      10


We depend on certain key executives, the loss of whom could disrupt our
operations, cause us to incur additional expenses and impede our ability to
expand our operations.

     Our success is dependent upon the efforts and abilities of our key
management personnel, particularly James C. New, our Chairman and Chief
Executive Officer, Brian C. Carr, our President, Gregory A. Marsh, our Vice
President and Chief Financial Officer, Alan Levin, M.D., our Chief Operating
Officer and Dennis M. Smith, Jr., M.D., our Senior Vice President and Medical
Director.  The services of these individuals would be very difficult to replace.
Therefore, it would be costly and time consuming to find suitable replacements
for these individuals.  The need to find replacements combined with the
temporary loss of these key services could also disrupt our operations and
impede our growth by diverting management attention away from our core business
and growth strategies.

Because of the complex nature of our billing and reimbursement arrangements, we
may be at a greater risk of Internal Revenue Service Examinations.

     The Internal Revenue Service, or IRS, conducted an examination of our
federal income tax returns for the tax years ended December 31, 1996 and 1997
and concluded that no changes to the tax reported needed to be made.  Although
we believe that we are in compliance with all applicable IRS rules and
regulations, if the IRS should determine that we are not in compliance in any
other years, we could be required to pay additional taxes, including penalties
and interest.  In addition, IRS examinations are costly in that they can consume
a great deal of management time and attention that would otherwise be spent
pursuing operational improvements and growth strategies.

Our stock price is volatile and the value of your investment may decrease for
various reasons, including reasons that are unrelated to the performance of our
business.

     There has been significant volatility in the market price of securities of
health care companies that often has been unrelated to the operating performance
of such companies.  In fact, our common stock, which trades on the Nasdaq
National Market, has traded from a low of $8 per share to a high of $26 15/16
per share for the year ended December 31, 2000.  We believe that various
factors, such as legislative and regulatory developments, quarterly variations
in our actual or anticipated results of operations, lower revenues or earnings
than those anticipated by securities analysts, the overall economy and the
financial markets could cause the price of our common stock to fluctuate
substantially.

          CAUTIONARY STATEMENT CONCERNING FORWARD-LOOKING STATEMENTS

     This prospectus contains forward-looking statements made pursuant to the
safe harbor provisions of the Private Securities Litigation Reform Act of 1995.
Statements contained anywhere in this prospectus that are not limited to
historical information are considered forward-looking statements within the
meaning of Section 27A of the Securities Act of 1933 and Section 21E of the
Securities Exchange Act of 1934, including statements regarding our
expectations, beliefs, intentions, plans or strategies regarding the future.
These forward-looking statements are based largely on our expectations and are
subject to a number of known and unknown risks and uncertainties discussed in
this prospectus and in other documents we file with the Securities and Exchange
Commission that may cause actual results to be materially different from those
anticipated, expressed or implied by the forward-looking statements. All
forward-looking statements included in this prospectus are based on information
available to us on the date hereof, and, except as required by law, we assume no
obligation to update any such forward-looking statements to reflect future
events or circumstances. Forward-looking statements are sometimes indicated by
words such as "may," "should," "believe," "expect," "anticipate" and similar
expressions.

                                      11


                                    USE OF PROCEEDS

     The shares of our common stock offered under this prospectus are for the
account of the selling stockholders.  We will not receive any proceeds from the
sale of common stock by the selling stockholders.  However, 5,085 of the shares
covered by this prospectus are subject to issuance by us pursuant to the
exercise of warrants held by some of the selling stockholders, 3,746 of which
have an exercise price of $0.12 per share and 1,339 of which have an exercise
price of $3.73 per share. We may receive cash proceeds from the exercise of
these warrants if the warrant holders elect not to make "cashless" exercises as
permitted under the terms of the warrants. Any cash proceeds that we receive
from the exercise of these warrants would be used for general corporate
purposes.

                                      12


                              SELLING STOCKHOLDERS

     The following table provides:

     .  The name of each of the selling stockholders;

     .  The number of shares of common stock beneficially owned by each selling
        stockholder before this offering;

     .  The number of shares of common stock being offered by each selling
        stockholder under this prospectus; and

     .  The number of shares of common stock beneficially owned by each selling
        stockholder after completion of the offering.

         The table assumes that the selling stockholders will sell all shares
they are offering under this prospectus, that the selling stockholders will not
acquire additional shares of our common stock prior to completion of this
offering, and that the selling stockholders will not dispose of any shares of
our common stock not covered by this prospectus. Each selling stockholder
beneficially owns less than 1% of the total number of shares of common stock
outstanding based on 25,195,344 shares of common stock outstanding as of June 5,
2001.




------------------------------------------------------------------------------------------------------
                                                    Shares                                  Shares
                                                  Beneficially                            Beneficially
                                                  Owned Before            Shares           Owned After
                 Name                               Offering              Offered           Offering
                 ----                               --------              -------           --------
------------------------------------------------------------------------------------------------------
                                                                                 
Haywood D. Cochrane, Jr.                               8,113                2,704                5,409
------------------------------------------------------------------------------------------------------
Brian C. Carr(1)                                      40,486               13,495               26,991
------------------------------------------------------------------------------------------------------
James E. Billington                                    8,676                2,892                5,784
------------------------------------------------------------------------------------------------------
William H. Brownie                                     4,498                1,499                2,999
------------------------------------------------------------------------------------------------------
Norman O. Hill                                         1,446                  482                  964
------------------------------------------------------------------------------------------------------
Douglas A. Olson                                       2,678                  893                1,785
------------------------------------------------------------------------------------------------------
Richland Ventures, L.P.                               29,607                9,869               19,738
------------------------------------------------------------------------------------------------------
Richland Ventures II, L.P.                            11,805                3,935                7,870
------------------------------------------------------------------------------------------------------
DFW Capital Partners, L.P.                            27,692                9,231               18,461
------------------------------------------------------------------------------------------------------
Calver Fund, Inc.                                     17,282                5,761               11,521
------------------------------------------------------------------------------------------------------
Noro - Moseley Partners III, L.P.                     17,193                6,731               11,462
------------------------------------------------------------------------------------------------------
J.G. Partnership, LTD.                                15,189                5,063               10,126
------------------------------------------------------------------------------------------------------
HLM Partners VII, L.P.                                13,905                4,635                9,270
------------------------------------------------------------------------------------------------------
HLM Partners V, L.P.                                  12,516                4,172                8,344
------------------------------------------------------------------------------------------------------
Chrysalis Ventures I, Ltd.                            11,522                3,841                7,681
------------------------------------------------------------------------------------------------------
SSM Venture Partners, L.P.                            11,462                3,821                7,641
------------------------------------------------------------------------------------------------------
J. David Grissom                                      11,143                3,714                7,429
------------------------------------------------------------------------------------------------------
Thomas McColl Chesney, M.D.                           12,378                4,126                8,252
------------------------------------------------------------------------------------------------------
Allen D. Berry III, M.D.  (2)                         12,940                4,313                8,627
------------------------------------------------------------------------------------------------------
Carolyn McIntyre Chesney, M.D.                        12,378                4,126                8,252
------------------------------------------------------------------------------------------------------
William A. Wesche, M.D.                                3,615                1,205                2,410
------------------------------------------------------------------------------------------------------
A. Weldon Schott, D.O.                                 1,232                  411                  821
------------------------------------------------------------------------------------------------------


                                      13



------------------------------------------------------------------------------------------------------
                                                                                      
Cheng C. Tsai, M.D.                                    1,003                  334                  669
------------------------------------------------------------------------------------------------------
Daniel J. Santa Cruz, M.D.                             2,276                  759                1,517
------------------------------------------------------------------------------------------------------
Eugene C. Wienke, M.D.                                 1,232                  411                  821
------------------------------------------------------------------------------------------------------
Kathryn DeSchryver, M.D.                                 546                  182                  364
------------------------------------------------------------------------------------------------------
Mark A. Hurt, M.D.                                       636                  212                  424
------------------------------------------------------------------------------------------------------
Oscar Lazcano, M.D.                                      546                  182                  364
------------------------------------------------------------------------------------------------------
Robert W. Brangle, M.D.                                1,232                  411                  821
------------------------------------------------------------------------------------------------------
Paul D. Cook, D.O.                                       318                  106                  212
------------------------------------------------------------------------------------------------------
Charles B. Bramlett Jr., M.D.                            803                  268                  535
------------------------------------------------------------------------------------------------------
Jack Teryle Pearson, M.D.                                803                  268                  535
------------------------------------------------------------------------------------------------------
Joseph C. Moore, M.D.                                  2,142                  714                1,428
------------------------------------------------------------------------------------------------------
H.W. Ferrell, M.D.                                     2,401                  800                1,601
------------------------------------------------------------------------------------------------------
John R. Olson, M.D.                                    2,401                  800                1,601
------------------------------------------------------------------------------------------------------
Carlene Ann Hawksley, 100% Trustee of
 the Hawksley Trust 1997 Created by
 Declaration of Trust, February 12,
 1997                                                  4,670                1,557                3,113
------------------------------------------------------------------------------------------------------
Kelly R. O'Keefe and Patricia O'Keefe,
 Trustee of the O'Keefe Family Trust
 dated December 20, 1995                               3,866                1,289                2,577
------------------------------------------------------------------------------------------------------
Robert M. Rinehart and Julie M.
 Rinehart as Trustee of the Rinehart
 Living Trust Dated November 2, 1989                   4,670                1,557                3,113
------------------------------------------------------------------------------------------------------
Simon S. Chan and Julia S. Chan 1993
 Intervivos Trust                                      4,670                1,557                3,113
------------------------------------------------------------------------------------------------------
Winterling Martin 1997 Revocable Trust                 4,670                1,557                3,113
------------------------------------------------------------------------------------------------------
Kenneth W. Westphal, M.D.                                803                  268                  535
------------------------------------------------------------------------------------------------------
William R. Beach, III                                    377                  126                  251
------------------------------------------------------------------------------------------------------
William H. West, M.D.                                  1,915                  638                1,277
------------------------------------------------------------------------------------------------------
William H. Lomicka                                     1,391                  464                  927
------------------------------------------------------------------------------------------------------
Wachovia Bank of Georgia, N.A. -
 Custodian for Alan I. Jacobson IRA                      377                  126                  251
------------------------------------------------------------------------------------------------------
UMB Bank, N.A., Trustee Melissa S.
 Elliot                                                   98                   33                   65
------------------------------------------------------------------------------------------------------
UMB Bank, N.A., Trustee Max L. Elliot,
 M.D.                                                     75                   25                   50
------------------------------------------------------------------------------------------------------
Thomas W. Beasley                                        377                  126                  251
------------------------------------------------------------------------------------------------------
Thomas L. West, M.D. Profit Sharing
 Trust                                                 1,842                  614                1,228
------------------------------------------------------------------------------------------------------
Steven F. Drake                                       20,083                6,694               13,389
------------------------------------------------------------------------------------------------------
Steffanie N. Drake Heritage Trust                        161                   54                  107
------------------------------------------------------------------------------------------------------
Samuel W. Bartholomew                                    461                  154                  307
------------------------------------------------------------------------------------------------------
Rose Marie Anderson Trust                              4,500                1,500                3,000
------------------------------------------------------------------------------------------------------
Robert R. West                                        37,861               12,620               25,241
------------------------------------------------------------------------------------------------------
Robert LaFollette West                                   444                  148                  296
------------------------------------------------------------------------------------------------------
Robert E. Brierty & Joan C. Brierty                      151                   50                  101
------------------------------------------------------------------------------------------------------
Robert Armistead First IRA Rollover                    3,954                1,318                2,636
------------------------------------------------------------------------------------------------------
Robert A. Frist                                          965                  322                  643
------------------------------------------------------------------------------------------------------


                                      14



------------------------------------------------------------------------------------------------------
                                                                                  
Nickel Medical Laboratory, Inc.                          342                  114                  228
------------------------------------------------------------------------------------------------------
Michael W. Blackburn                                      38                   13                   25
------------------------------------------------------------------------------------------------------
Melissa D. Springer                                      117                   39                   78
------------------------------------------------------------------------------------------------------
McKenzie Investment Company                            2,167                  722                1,445
------------------------------------------------------------------------------------------------------
Mark T. Springer                                         117                   39                   78
------------------------------------------------------------------------------------------------------
Marianna L. Dennison                                   1,712                  571                1,141
------------------------------------------------------------------------------------------------------
Kristine Margaret West                                   444                  148                  296
------------------------------------------------------------------------------------------------------
Trico & Co.                                            1,285                  428                  857
------------------------------------------------------------------------------------------------------
Jackson W. and Elizabeth W. Moore                      4,788                1,596                3,192
------------------------------------------------------------------------------------------------------
Jack Roy Anderson Trust Fund                           4,500                1,500                3,000
------------------------------------------------------------------------------------------------------
Jack R. Anderson                                       4,356                1,452                2,904
------------------------------------------------------------------------------------------------------
Gerald M. Bordin & Sheila W. Bordin                      188                   63                  125
------------------------------------------------------------------------------------------------------
Frederick C. Glavin & Martha G. Glavin                   377                  126                  251
------------------------------------------------------------------------------------------------------
Ernest S. Tucker, III                                    151                   50                  101
------------------------------------------------------------------------------------------------------
Douglas Crawford Huber                                   226                   75                  151
------------------------------------------------------------------------------------------------------
Delaware Charter Guarantee & Trust -
 FBO Robert Nakamura IRA                                 151                   50                  101
------------------------------------------------------------------------------------------------------
Dawn Dixie Drake Heritage Trust                          161                   54                  107
------------------------------------------------------------------------------------------------------
Cora S. Humberson & Michael Whittaker                     90                   30                   60
------------------------------------------------------------------------------------------------------
Brian Datnow, M.D.                                       377                  126                  251
------------------------------------------------------------------------------------------------------
Bobbye Williams                                          151                   50                  101
------------------------------------------------------------------------------------------------------
Arthur S. Demoss Foundation                            7,141                2,380                4,761
------------------------------------------------------------------------------------------------------
Andres Aquino                                            151                   50                  101
------------------------------------------------------------------------------------------------------
James R. Miller Lifetime Trust                           471                  157                  314
------------------------------------------------------------------------------------------------------
Vincent H. Stack Living Trust                            188                   63                  125
------------------------------------------------------------------------------------------------------
Stephen M. Russell, M.D.                                 161                   54                  107
------------------------------------------------------------------------------------------------------
Rodgers Business Interests                               461                  154                  307
------------------------------------------------------------------------------------------------------
ABS Capital Partners II, LP                          601,471              200,490              400,981
------------------------------------------------------------------------------------------------------
James M. Shapiro and Sarah B. Shapiro
 Trustee FBO James M. Shapiro and
 Sarah B. Shapiro Trust                                1,004                  335                  669
------------------------------------------------------------------------------------------------------
Union Street Partners, L.P.                           40,165               13,388               26,776
------------------------------------------------------------------------------------------------------
Questor Partners Fund, L.P.                          701,166              233,722              467,444
------------------------------------------------------------------------------------------------------
Questor Side-by-Side Partners, L.P.                   50,328               16,776               33,552
------------------------------------------------------------------------------------------------------
Robert J. Friedman, M.D.                             113,625               37,875               75,750
------------------------------------------------------------------------------------------------------
Edward Heilman, M.D.                                 113,625               37,875               75,750
------------------------------------------------------------------------------------------------------
Richard Jacoby, M.D.                                  70,644               20,252               50,392
------------------------------------------------------------------------------------------------------
Mario DiLeonardo, M.D.                                70,644               20,252               50,392
------------------------------------------------------------------------------------------------------
Waine C. Johnson, M.D.                                29,664                9,888               19,776
------------------------------------------------------------------------------------------------------
Thomas D. Griffin, M.D.                               29,664                9,888               19,776
------------------------------------------------------------------------------------------------------
Gary R. Kantor, M.D.                                  29,664                9,888               19,776
------------------------------------------------------------------------------------------------------
Richard L. Spielvogel, M.D.                           29,664                9,888               19,776
------------------------------------------------------------------------------------------------------
Finova Mezzanine Capital Inc.                         21,034                7,011               14,023
------------------------------------------------------------------------------------------------------
Ben F. Martin, M.D.                                   20,113                6,704               13,409
------------------------------------------------------------------------------------------------------
John H. Parker, M.D.                                  20,113                6,704               13,409
------------------------------------------------------------------------------------------------------
Roxanne Perryman, M.D.                                 1,607                  536                1,071
------------------------------------------------------------------------------------------------------
George F. Bale, M.D.                                  12,137                4,046                8,091
------------------------------------------------------------------------------------------------------


                                      15



------------------------------------------------------------------------------------------------------
                                                                                  
Robert M. Bradley, M.D.                               12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Michael F. Bugg, M.D.                                 12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Thomas R. Callihan, M.D.                              12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Kenneth D. Groshart, M.D.                             12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Johnnie Cameron Hall, M.D.                            12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Shamim M. Moinuddin, M.D.                             12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Gene D. Spencer, Jr., M.D.                            12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Bruce L. Webber, M.D.                                 12,137                4,046                8,091
------------------------------------------------------------------------------------------------------
Richard C. Olshock, M.D.                              12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
James A. Hopfenbeck, M.D.                             12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
Cathy Van Blerkom, M.D.                                3,088                1,029                2,059
------------------------------------------------------------------------------------------------------
John E. Boline, M.D.                                   6,176                2,059                4,117
------------------------------------------------------------------------------------------------------
Mary E. Corkhill, M.D.                                12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
William F. Cox, Jr., M.D.                             12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
Donald K. McClure, M.D.                                8,235                2,745                5,490
------------------------------------------------------------------------------------------------------
Timothy W. Morgan, M.D.                               12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
Steven J. Temple, M.D.                                12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
Carlton L. Wallis, Jr., M.D.                           6,176                2,059                4,117
------------------------------------------------------------------------------------------------------
Michael J. Pushchak, M.D.                             12,353                4,118                8,235
------------------------------------------------------------------------------------------------------
Craig MacNab(3)                                        1,606                  535                1,071
------------------------------------------------------------------------------------------------------
Robert A. Reeves(3)                                      803                  268                  535
------------------------------------------------------------------------------------------------------
H. Richard Pascoe, M.D.(3)                               321                  107                  214
------------------------------------------------------------------------------------------------------
Russell T. Ray(3)                                        803                  268                  535
------------------------------------------------------------------------------------------------------
Gerardo Rosencranz(3)                                    481                  160                  321
------------------------------------------------------------------------------------------------------
Stuart F. Smith(3)                                       321                  107                  214
------------------------------------------------------------------------------------------------------
R. Riley Sweat(3)                                        321                  107                  214
------------------------------------------------------------------------------------------------------
David Wilson(3)                                          481                  160                  321
------------------------------------------------------------------------------------------------------
Guarantee and Trust F/B/O. Edward S.
 Brokaw(3)                                             1,606                  535                1,071
------------------------------------------------------------------------------------------------------
SunTrust Equitable Securities
 Corporation(3)                                        8,032                2,678                5,354
------------------------------------------------------------------------------------------------------
Mark R. Klausner(3)                                      160                   53                  107
------------------------------------------------------------------------------------------------------


_________________________
(1)  Shareholdings include 321 shares issuable pursuant to the exercise of
     warrants, 107 of which are being offered pursuant to this prospectus.

(2)  Shareholdings include 803 shares owned jointly with Dr. Berry's spouse,
     Dianne J. Berry.

(3)  Shareholdings represent shares issuable pursuant to the exercise of
     warrants.

Certain Relationships among the Selling Stockholders and AmeriPath

     We acquired Inform DX on November 30, 2000 in a stock-for-stock merger
transaction. Each of the selling stockholders was a stockholder or warrant
holder of Inform DX at the time of the merger. The shares covered by this
prospectus were issued pursuant to the merger or are issuable pursuant to
warrants assumed by us in connection with the merger.

     In connection with the Inform DX merger, AmeriPath agreed to take certain
actions necessary to register the resale of the shares covered by this
prospectus, including the preparation and filing of the registration statement
of which this prospectus forms a part and the payment of expenses associated
with the registration statement and this prospectus.

                                      16


     Some of the selling stockholders were employees, officers or directors of
Inform DX prior to the merger.

         Some of the selling stockholders currently are employees of AmeriPath
or of medical practices managed by AmeriPath. Some of the Selling Stockholders
are officers, directors or owners of medical practices managed by
AmeriPath.

     Brian C. Carr is the President of AmeriPath and James E. Billington is the
Senior Vice President, Operations of AmeriPath.

     Brian C. Carr is also a director of AmeriPath.

                              PLAN OF DISTRIBUTION

       Our common stock is quoted on the Nasdaq National Market under the symbol
"PATH".  This prospectus is intended to be used to comply with the prospectus
delivery requirements of the Securities Act of 1933 in connection with any
offers or resales. Any or all of the shares of our common stock offered under
this prospectus may be sold from time to time by the selling stockholders, or by
pledgees, donees, transferees, or other successors in interest.  These sales may
be made:

     .   to or through underwriters, agents, brokers or dealers;
     .   directly to one or more purchasers;
     .   through agents on a best efforts basis; or
     .   through a combination of any such methods of sale.

     In addition, such sales may be made in the over-the-counter market, or
otherwise at prices and at terms then prevailing or at prices related to the
then current market price or in negotiated transactions.  Any or all of the
shares of common stock may be sold by one or more of the following:

     .   a block trade in which the broker or dealer so engaged will attempt to
         sell the shares as agent but may position and resell a portion of the
         block as principal to facilitate the transaction;
     .   purchases by a broker or dealer as principal and resale by such broker
         or dealer for its account pursuant to this prospectus;
     .   an exchange distribution in accordance with the rules of the exchange
         or any automated interdealer quotation system on which the common stock
         is then listed;
     .   ordinary brokerage transactions and transactions in which the broker
         solicits purchasers; and
     .   writing options on the shares.

     Any underwriters, agents or broker-dealers involved in the distribution
of the shares may receive compensation in the form of discounts, concessions or
commissions from the selling stockholders and/or the purchasers of the shares
for which such underwriters, agents or broker-dealers may act as agents or to
whom they sell as principals, or both (which compensation as to an underwriter,
agent or particular broker-dealer will be negotiated prior to the sale and may
be in excess of customary compensation).  If required by applicable law at the
time a particular offer of shares is made, the terms and conditions of that
transaction will be set forth in a supplement to this prospectus.

     The selling stockholders and any underwriters, agents or broker-dealers who
act in connection with the sale of the shares under this prospectus may be
deemed to be "underwriters" within the meaning of Section 2(11) of the
Securities Act, and any compensation received by them might be deemed to be
underwriting discounts and commissions under the Securities Act.

                                      17


     The selling stockholders will pay all applicable stock transfer taxes,
transfer fees and brokerage commissions or underwriting or other discounts. We
will bear all expenses in connection with the registration of the shares being
offered by the selling stockholders. We have agreed to indemnify the selling
stockholders against certain liabilities, including liabilities under the
Securities Act.


                                 LEGAL MATTERS

     Alston & Bird LLP, Atlanta, Georgia, will pass upon the status of the
shares offered under this prospectus as legally and validly issued, fully paid
and nonassessable.

                                    EXPERTS

     The financial statements of AmeriPath, Inc. and its consolidated
subsidiaries, as of December 31, 2000 and 1999 and for each of the three years
in the period ended December 31, 2000, except Pathology Consultants of America,
Inc. (d/b/a "InformDX") as of December 31, 1999 and for the years ended December
31, 1999 and 1998, incorporated by reference in this prospectus have been
audited by Deloitte & Touche LLP as stated in their reports incorporated by
reference herein. The financial statements of InformDX, consolidated with those
of AmeriPath, Inc. and not presented separately herein, have been audited by
Ernst & Young LLP as stated in their reports incorporated by reference herein.
Such financial statements of the Company and its consolidated subsidiaries are
incorporated by reference herein in reliance upon the respective reports of such
firms given upon their authority as experts in accounting and auditing. All of
the foregoing firms are independent auditors.

                      WHERE YOU CAN FIND MORE INFORMATION

     We file annual, quarterly and current reports, proxy statements and other
information with the Securities and Exchange Commission. These reports, proxy
statements and other information may be obtained:

     .  At the public reference room of the Commission, Room 1024 -- Judiciary
        Plaza, 450 Fifth Street, N.W., Washington, D.C. 20549;
     .  At the public reference facilities at the Commission's regional offices
        located at Seven World Trade Center, 13th Floor, New York, New York
        10048 or Northwestern Atrium Center, 500 West Madison Street, Suite
        1400, Chicago, Illinois 60661;
     .  From the Commission, Public Reference Room, Judiciary Plaza, 450 Fifth
        Street, N.W., Washington, D.C.  20549;
     .  At the offices of The Nasdaq Stock Market, Inc., Reports Section, 1735 K
        Street, N.W., Washington, D.C.  20006; or
     .  From the internet site maintained by the Commission at
        http://www.sec.gov, which contains reports, proxy and information
        ------------------
        statements and other information regarding issuers that file
        electronically with the Commission.

     Some locations may charge prescribed rates or modest fees for copies.  For
more information on the public reference room, call the Commission at 1-800-SEC-
0330.

     We filed with the Securities and Exchange Commission a registration
statement on Form S-3 (which contains this prospectus) under the Securities Act
of 1933, as amended, to register with the Securities and Exchange Commission the
resale by the selling stockholders of our common stock. This prospectus does not
contain all the information you can find in the registration statement or the
exhibits

                                      18


and schedules to the registration statement. For further information with
respect to us, and our common stock, please refer to the registration statement,
including the exhibits and schedules. You may inspect and copy the registration
statement, including the exhibits and schedules, as described above.

                INCORPORATION OF CERTAIN DOCUMENTS BY REFERENCE

     The Securities and Exchange Commission allows us to "incorporate by
reference" the information that we file with them in other documents, which
means that we can disclose important information to you by referring to those
documents. The information incorporated by reference is considered to be part of
this prospectus, and later information that we file with the Securities and
Exchange Commission will automatically update and supersede this information. We
incorporate by reference the documents listed below and all future documents
filed with the Securities and Exchange Commission under Sections 13(a), 13(c),
14, or 15(d) of the Securities Exchange Act of 1934 until the termination of the
offering to which this prospectus relates:

     .  Current Report on Form 8-K, filed March 6, 2001;

     .  Current Report on Form 8-K, filed April 6, 2001;

     .  Annual Report on Form 10-K for the year ended December 31, 2000, filed
        April 2, 2001, including those portions of our proxy statement for our
        2001 annual meeting of stockholders that are incorporated into the Form
        10-K by reference;

     .  Quarterly Report on Form 10-Q for the quarter ended March 31, 2001,
        filed May 15, 2001;

     .  The description of common stock set forth in our registration statement
        filed pursuant to Section 12 of the Exchange Act, and any amendment or
        report filed for the purpose of updating such description; and

     .  The description of rights to purchase Series A Junior Participating
        Preferred Stock set forth in our registration statement filed pursuant
        to Section 12 of the Exchange Act, and any amendment or report filed for
        the purpose of updating such description.

     On written or oral request, we will provide at no cost to each person who
receives a copy of this prospectus, a copy of any or all of the documents
incorporated in this prospectus by reference. We will not provide exhibits to
any of the documents listed above, however, unless those exhibits are
specifically incorporated by reference into those documents. You should direct
your request to the Secretary of AmeriPath, 7289 Garden Road, Suite 200, Riviera
Beach, Florida 33404, telephone number (561) 845-1850.

     You should rely only on the information that we incorporate by reference or
provide in this prospectus or any supplement. We have not authorized anyone else
to provide you with different information. Neither we nor the selling
stockholders will make an offer of these shares in any state where the offer is
not permitted. You should not assume that the information in this prospectus or
any supplement is accurate as of any date other than the date on the front of
those documents.

                                      19


                                    PART II

                    INFORMATION NOT REQUIRED IN PROSPECTUS

ITEM 14. OTHER EXPENSES OF ISSUANCE AND DISTRIBUTION


Registration fee to Securities and Exchange Commission............  $ 4,987
Accounting fees and expenses......................................        0
Legal fees and expenses...........................................    5,000
Miscellaneous expenses............................................    2,000

     Total........................................................  $11,987
                                                                    =======

The foregoing items, except for the registration fee to the Securities and
Exchange Commission, are estimated. We have agreed to bear all expenses in
connection with the registration of the shares being offered by the selling
stockholders, except that the selling stockholders will bear all underwriting
discounts and commissions and transfer taxes, if any.  We have agreed to
indemnify the selling stockholders against certain liabilities, including
liabilities under the Securities Act.

ITEM 15. INDEMNIFICATION OF DIRECTORS AND OFFICERS

       Our amended and restated certificate of incorporation eliminates the
personal liability of our directors to AmeriPath and its stockholders for
monetary damages for breach of fiduciary duty as a director, except that it does
not eliminate the liability of a director:

       .  for any breach of the duty of loyalty to AmeriPath and its
          stockholders;

       .  for acts or omissions not in good faith which involve intentional
          misconduct or a knowing violation of law;

       .  under the Delaware General Corporation Law for a director's willful or
          negligent violation of statutory provisions that prevent the unlawful
          payment of a dividend; and

       .  for any transaction in which a director receives an improper personal
          benefit.

       In addition, if at any time the Delaware General Corporation Law is
amended to authorize further elimination or limitation of the personal liability
of a director, then the liability of each of our directors shall be eliminated
or limited to the fullest extent permitted by such provisions, as so amended,
without further action by the stockholders, unless otherwise required.

       Our bylaws require us to indemnify any person who was or is a party or is
threatened to be made a party to any threatened, pending or completed action,
suit or proceeding (other than an action by or in the right of AmeriPath) by
reason of the fact that the indemnified person was or is a director, officer,
employee or agent of AmeriPath, or is or was serving at the request of AmeriPath
as a director, officer, employee or agent of another corporation, partnership,
joint venture, trust or other enterprise against expenses, judgments, fines and
amounts paid in settlement actually and reasonably incurred by such indemnified
person, as long as the indemnified person:

       .  acted in good faith;

                                     II-1


       .  acted in a manner reasonably believed to be in or not opposed to the
          best interests of AmeriPath; and

       .  with respect to any criminal action or proceeding, had no reasonable
          cause to believe his or her conduct was unlawful.

       Our bylaws also require us to indemnify any person who was or is a party
or is threatened to be made a party to any threatened, pending or completed
action or suit by or in the right of AmeriPath to procure a judgment in
AmeriPath's favor by reason of the fact that the indemnified person was or is a
director, officer, employee or agent of AmeriPath, or is or was serving at the
request of AmeriPath as a director, officer, employee or agent of another
corporation, partnership, joint venture, trust or other enterprise against
expenses, judgments, fines and amounts paid in settlement actually and
reasonably incurred by such indemnified person, as long as the indemnified
person:

       .  acted in good faith; and

       .  acted in a manner reasonably believed to be in or not opposed to the
          best interests of AmeriPath.

       However, no indemnification shall be made by us under the preceding
paragraph in respect of any claim, issue or matter as to which such person shall
have been adjudged to be liable for negligence or misconduct in the performance
of his or her duty to us unless otherwise determined by court order.

       The determination of whether the applicable standard of conduct described
above has been meet shall be made by:

       .  our Board of Directors by a majority vote of a quorum consisting of
          disinterested directors; or

       .  if such a quorum is not obtainable, or, even if obtainable, a quorum
          of disinterested directors so directs, by independent legal counsel in
          a written opinion; or

       .  by the stockholders.

       AmeriPath maintains a standard form of officers' and directors' liability
insurance policy that provides coverage to its officers and directors for
certain liabilities, including certain liabilities that may arise out of this
registration statement.

       We have agreed to indemnify each selling stockholder, each underwriter,
if any, and each person controlling the selling stockholders or the
underwriters, if any, within the meaning of the Securities Act, from and against
any losses, claims, damages or liabilities, joint or several, to which such
selling stockholders, underwriters or controlling persons may become subject
under the Securities Act or otherwise, insofar as such losses, claims, damages
or liabilities (or actions in respect thereof) arise out of or are based upon
any untrue statement or alleged untrue statement of any material fact contained
in this prospectus, or arise out of or are based upon the omission or alleged
omission to state therein a material fact required to be stated therein or
necessary to make the statements therein not misleading, or any violations by us
of applicable federal or state securities laws relating to such registration.

                                     II-2


ITEM 16. EXHIBITS AND FINANCIAL STATEMENT SCHEDULES

(a) Exhibits

EXHIBIT
NUMBER                   DESCRIPTION
--------

2.1            Agreement and Plan of Merger by and among the Registrant, AMP
               Merger Corp. and Pathology Consultants of America, Inc. (d/b/a
               Inform DX), dated as of November 7, 2000 (incorporated by
               reference from Exhibit 2.2 to our Annual Report on Form 10-K for
               the fiscal year ended December 31, 2000)

4.1            Amended and Restated Certificate of Incorporation (incorporated
               by reference from Exhibit 3.1 to our registration statement on
               Form S-1, Registration No. 333-34265)

4.2            Amended and Restated Bylaws (incorporated by reference from
               Exhibit 3.2 to our registration statement on Form S-1,
               Registration No. 333-34265)

4.3            Certificate of Amendment to the Amended and Restated Certificate
               of Incorporation (incorporated by reference from Exhibit 3.3 to
               our registration statement on Form S-1, Registration No. 333-
               34265)

4.4            Rights Agreement, dated as of April 8, 1999, between the
               Registrant and American Stock Transfer & Trust Company, as Rights
               Agent including the form of Certificate of Designations of Series
               A Junior Participating Preferred Stock, the form of Rights
               Certificate, and the form of Summary of Rights (incorporated by
               reference to Exhibit 4.1 to our Current Report on Form 8-K filed
               April 16, 1999)

4.5            Registration Rights Agreement, dated November 30, 2000, among the
               Registrant and the Shareholders and Warrant Holders of Pathology
               Consultants of America, Inc. (d/b/a Inform DX) (incorporated by
               reference from Exhibit 10.46 to our Annual Report on Form 10-K
               for the fiscal year ended December 31, 2000)

5.1            Opinion of Alston & Bird LLP, including consent

23.1           Consent of Deloitte & Touche LLP

23.2           Consent of Ernst & Young LLP

23.3           Consent of Alston & Bird LLP (filed as part of Exhibit 5.1)

24.1           Power of Attorney (included as part of the signature page to this
               registration statement, as filed on April 20, 2001)

(b) Financial Statement Schedules

ITEM 17. UNDERTAKINGS

      The undersigned registrant hereby undertakes:

                                     II-3


     (1) To file, during any period in which offers or sales are being
made, a post-effective amendment to this Registration Statement:

            (i)   To include any prospectus required by Section 10(a)(3) of the
Securities Act of 1933;

            (ii)  To reflect in the prospectus any facts or events arising after
the effective date of the Registration Statement (or the most recent post-
effective amendment thereof) which, individually or in the aggregate, represent
a fundamental change in the information set forth in the Registration Statement.
Notwithstanding the foregoing, any increase or decrease in volume of securities
offered (if the total dollar value of securities offered would not exceed that
which was registered) and any deviation from the low or high end of the
estimated maximum offering range may be reflected in the form of prospectus
filed with the Commission pursuant to Rule 424(b) if, in the aggregate, the
changes in volume and price represent no more than a 20% change in the maximum
aggregate offering price set forth in the "Calculation of Registration Fee"
table in the effective registration statement.

            (iii) To include any material information with respect to the plan
of distribution not previously disclosed in the Registration Statement or any
material change to such information in the Registration Statement.

            provided, however, that paragraphs (a)(1)(i) and (a)(1)(ii) of this
Section do not apply if the registration statement is on Form S-3, Form S-8 or
Form F-3 and the information required to be included in a post-effective
amendment by those paragraphs is contained in periodic reports filed with or
furnished to the Commission by the registrant pursuant to Section 13 or 15(d) of
the Securities Exchange Act of 1934 that are incorporated by reference in the
registration statement.

     (2) That, for the purpose of determining any liability under the
Securities Act, each such post-effective amendment shall be deemed to be a new
Registration Statement relating to the securities offered therein, and the
offering of such securities at that time shall be deemed to be the initial bona
fide offering thereof.

     (3) To remove from registration by means of a post-effective amendment
any of the securities being registered which remain unsold at the termination of
the offering.

     The undersigned registrant hereby further undertakes that, for purposes of
determining any liability under the Securities Act of 1933, each filing of the
registrant's annual report pursuant to Section 13(a) or Section 15(d) of the
Exchange Act (and, where applicable, each filing of an employee benefit plan's
annual report pursuant to Section 15(d) of the Exchange Act) that is
incorporated by reference in the Registration Statement shall be deemed to be a
new registration statement relating to the securities offered therein, and the
offering of such securities at that time shall be deemed to be the initial bona
fide offering thereof.

     Insofar as indemnification for liabilities arising under the Securities Act
of 1933 may be permitted to directors, officers and controlling persons of the
registrant pursuant to the foregoing provisions, or otherwise, the registrant
has been advised that in the opinion of the Securities and Exchange Commission
such indemnification is against public policy as expressed in the Securities Act
of 1933 and is therefore, unenforceable. In the event that a claim for
indemnification against such liabilities (other than the payment by the
registrant of expenses incurred or paid by a director, officer or controlling
person of the registrant in the successful defense of any action, suit or
proceeding) is asserted by such director, officer or controlling person in
connection with the securities being registered, the registrant will, unless in
the opinion of its counsel the matter has been settled by controlling precedent,
submit to a

                                     II-4


court of appropriate jurisdiction the question whether such indemnification by
it is against public policy as expressed in the Securities Act and will be
governed by the final adjudication of such issue.

       The undersigned registrant hereby undertakes that:

       (1) For purposes of determining any liability under the Securities Act of
1933, the information omitted from the form of prospectus filed as part of this
Registration Statement in reliance upon Rule 430A and contained in a form of
prospectus filed by the registrant pursuant to Rule 424(b)(1) or (4), or 497(h)
under the Securities Act of 1933 shall be deemed to be part of this Registration
Statement as of the time it was declared effective.

       (2) For the purpose of determining any liability under the Securities Act
of 1933, each post-effective amendment that contains a form of prospectus shall
be deemed to be a new registration statement relating to the securities offered
therein, and the offering of such securities at that time shall be deemed to be
the initial bona fide offering thereof.

                                     II-5


                                  SIGNATURES

     Pursuant to the requirements of the Securities Act of 1933, the registrant
certifies that it has reasonable grounds to believe that it meets all of the
requirements for filing on Form S-3 and has duly caused this registration
statement to be signed on its behalf by the undersigned, thereunto duly
authorized, in the City of Riviera Beach, State of Florida on the 8th day of
June, 2001.

                              AmeriPath, Inc.

                              By: /s/ James C. New
                                 ------------------------------------
                              Name: James C. New
                              Title: Chairman and Chief Executive Officer

          Pursuant to the requirements of the Securities Act of 1933, this
registration statement has been signed by the following persons in the
capacities and on the dates indicated.



                  Signature                                 Title                         Date
                  ---------                                 -----                         ----
                                                                              
/s/ James C. New                               Chairman and Chief Executive         June 8, 2001
-----------------------------------------      Officer(Principal Executive
James C. New                                   Officer)

/s/ Gregory A Marsh                            Vice President, Chief Financial      June 8, 2001
-----------------------------------------      Officer and Secretary
Gregory A. Marsh                               (Principal Financial and
                                               Accounting Officer)

              *                                Director                             June 8, 2001
-----------------------------------------
Brian C. Carr

              *                                Director                             June 8, 2001
-----------------------------------------
E. Martin Gibson

              *                                Director                             June 8, 2001
-----------------------------------------
Alan Levin, M.D.

              *                                Director                             June 8, 2001
-----------------------------------------
C. Arnold Renschler, M.D.

              *                                Director                             June 8, 2001
-----------------------------------------
E. Roe Stamps, IV




*By:  /s/ James C. New
    ------------------------
       James C. New
       Attorney-in-Fact

                                     II-6


                                 EXHIBIT INDEX

EXHIBIT
NUMBER         DESCRIPTION

2.1            Agreement and Plan of Merger by and among the Registrant, AMP
               Merger Corp. and Pathology Consultants of America, Inc. (d/b/a
               Inform DX), dated as of November 7, 2000 (incorporated by
               reference from Exhibit 2.2 to our Annual Report on Form 10-K for
               the fiscal year ended December 31, 2000)

4.1            Article II of the Articles of Incorporation, as amended
               (incorporated by reference from Exhibit 3.1 to our registration
               statement on Form S-1, Registration No. 333-34265)

4.2            Amended and Restated Bylaws (incorporated by reference from
               Exhibit 3.2 to our registration statement on Form S-1,
               Registration No. 333-34265)

4.3            Certificate of Amendment to the Amended and Restated Certificate
               of Incorporation (incorporated by reference from Exhibit 3.3 to
               our registration statement on Form S-1, Registration No. 333-
               34265)

4.4            Rights Agreement, dated as of April 8, 1999, between the
               Registrant and American Stock Transfer & Trust Company, as Rights
               Agent including the form of Certificate of Designations of Series
               A Junior Participating Preferred Stock, the form of Rights
               Certificate, and the form of Summary of Rights (incorporated by
               reference to Exhibit 4.1 to our Current Report on Form 8-K filed
               April 16, 1999)

4.5            Registration Rights Agreement, dated November 30, 2000, among the
               Registrant and the Shareholders and Warrant Holders of Pathology
               Consultants of America, Inc. (d/b/a Inform DX) (incorporated by
               reference from Exhibit 10.46 to our Annual Report on Form 10-K
               for the fiscal year ended December 31, 2000)

5.1            Opinion of Alston & Bird LLP, including consent

23.1           Consent of Deloitte & Touche LLP

23.2           Consent of Ernst & Young LLP

23.3           Consent of Alston & Bird LLP (filed as part of Exhibit 5.1)

24.1           Power of Attorney (included as part of the signature page to this
               registration statement, as filed on April 20, 2001)